SCIENCE SERVICES Tipps and Tricks for using LR White Sections
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Technical Tips......................................................................................................................................................1 Using LR White for Light Microscopy..................................................................................................................2 Using LR White for Hard Tissue..........................................................................................................................3 Using LR White for Electron Microscopy............................................................................................................5 Using LR White for Immunohistochemistry.........................................................................................................7 Using LR White for Electron Microscopic Immunocytochemistry.......................................................................9
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LR White is preferable to Unicryl for immunogold detection of fixation-sensitive nuclear antigens.
The purpose of this study was to compare two electron microscopy embedding media - LR White and Unicryl - with regard to cell morphologyical and immunohistochemical preservation properties for the study of fixation-sensitive nuclear antigens. Human cervical carcinoma (HeLa) cells were fixed with 2% paraformaldehyde and 0.1% glutaraldehyde, and embedded in parallel in the two resins: LR White an...
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متن کاملVisualization of identified GFP-expressing cells by light and electron microscopy.
We have developed a procedure for visualizing GFP expression in fixed tissue after embedding in LR White. We find that GFP fluorescence survives fixation in 4% paraformaldehyde/0.1% glutaraldehyde and can be visualized directly by fluorescence microscopy in unstained, 1 microm sections of LR White-embedded material. The antigenicity of the GFP is retained in these preparations, so that GFP loca...
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Pulmonary surfactant stabilizes the lung by reducing surface tension at the air-water interface of the alveoli. Surfactant is present in the lung in a number of morphological forms, including tubular myelin (TM). TM is composed of unusual 40 x 40 nm square elongated proteolipid tubes. Atomic force microscopy (AFM) was performed on polymer-embedded Lowicryl and London Resin-White (LR-White) unst...
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